Bial's Test - Qualitative Test in Carbohydrates


In this Biochemistry laboratory test, the Bial's Test procedure is demonstrated. The lesson covers the description of the laboratory instruments and materials that are used in such tests and the test procedure.


The objective of these experiments is to augment the understanding of basic principles of drug biological response. At the end of the lesson, students should be able to describe the basis and principles of Bial's test and use the qualitative test to distinguish between pentoses and hexoses.

Author(s): George KoffuorView Details of Author
Year: 2012
Keywords: Bial's test, pentoses, hexoses, bial's reagent, qualitative test
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Bial's reagent contains orcinol, conc HCL, and FeCL3. Oricinol form a blue-green coloured condensation product with furfural generated by the dehydration of pentoses and pentosans. Bial's test is a test to distinguish pentoses from hexoses. Hexoses, which form 5-hydroxymethyl furfural, yield a yellow to brown colour.




Describe the basis and principles of Bial's test.

Use the qualitative test to distinguish between pentoses and hexoses.


Lesson Content

Bial's Test (video)
Bial's Test





Dr. George Koffuor
Department of Pharmacology
College of Health Sciences - KNUST
Kumasi, Ghana

Student Volunteer

Stephen Fletchyann
Department of Communication Design
Kumasi, Ghana


OER Technical Support




Benjamin Prempeh
OER Media Specialist
KNUST, Kumasi - Ghana

John Kofi Dogbey
Media Specialist
KNUST,Kumasi - Ghana

Voice Over

ella kasanga

Ella Kasanga
Kumasi, Ghana




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