Courses Under Pharmacology

Strychnine Poisoning in Toads

This pharmacological laboratory procedure demonstrates the Central Nervous System excitatory effect of Strychnine in toads. A dose of Strychnine is injected into the ventral lymph node of the toad then the hyper-stimulation of the Strychnine dosage is observed.   Stychnine acts as a selective competitive antagonist to block the inhibitory effects of glycine at all glycine receptors. Strychn...

View Course

Frog Heart Preparation

This laboratory procedure module explains the process of isolating and mounting a frog heart for the study of cardiac cell excitation. It includes a video demonstration of the procedure to guide student to be able to understand, and on his own, isolate and mount a tissue without supervision at the laboratory.

View Course

Autonomic Effect After Muscarinic Hyper-Activity

In this laboratory procedure, the video demonstration seeks to teach on the effect of muscarinic agonists and its parasympathetic effects in rats. This resources should guide you to be able to perform such procedure in the laboratory and observe the lab animals for the results.   Thus, in a succeful procedure, the effects of lacrimation, salivation, defaecation, urination, pilo-erection, rh...

View Course

The Harvard Kymograph

The Harvard Kymograph with stimulator will be the focus of this resource.  Setting up this equipment in the appropriate manner is important in order to obtain the best results in an experiment. The video lesson will demonstrate the setting up of the Harvard Kymograph with all possible adjustments that can be made for the best results.   This equipment enables the recording of...

View Course

Maintenance of an Isolated Smooth Muscle Preparation In Vitro

This course would look at maintaning an isolated smooth muscle prepared in vitro, that is, outside the body. The Harvard Kymograph with stimulator is the equipment that enables the recording of contractions of muscles from the isolated tissue preparation. The video lessons highlight the conditions neccessary for the maintainance of a smooth muscle prepation.   Our objective is to ...

View Course

Set up an Isolated Tissue-organ Bath Experiment

The Setting Up of an Organ Bath would be the topic of this laboratory procedure. This course covers the organ bath equipment and the procedure in adjusting for isolated tissue preparations. The video lessons will serve as a guide for students to be able to identify the components and functions of the isolated tissue/organ bath. Another objective would focus on adjusting the equipment sui...

View Course

Respiratory Depressant Effect of Morphine in Rabbits

The principle of the laboratory procedure would be to demonstrate the respiratory and miotic effect of morphine in rabbits. With morphine as a benchmark of analgesics in clinical medicine, it is important to understand how it acts directly on the central nervous system to relieve pain. However, one of the main adverse effects of morphine is respiratory depression. Also, morphine causes miosis. &n...

View Course

Respiratory Depressant Effect of Pentobarbitone in Rabbits

In this course, the experiment captured in the video would demonstrate the respiratory depressant effect of pentobarbitone. With this as our objective, the laboratory procedure would explain this by observing the effect of pentobarbitone in an induced rabbit.Pentobarbitone, as a non-selective central nervous system depressant, can produce effects ranging from sedation, reduction of anxiety to unco...

View Course

Haematology - The White Blood Cell Count

This programme demonstrates in a video lesson the procedure in determining whether there is adequate number of white blood cell in circulation. The procedure covers the description of the laboratory instruments and materials that are used in such tests.   At the end of the procedure, an observation of the test result is made and interpreted with the use of a microscope.

View Course

Haematology - The Red Blood Cell Count

This programme demonstrates in a video lesson the procedure in determining whether there is adequate number of red blood cell in circulation. The procedure covers the description of the laboratory instruments and materials that are used in such tests.   At the end of the procedure, an observation of the test result is made and interpreted with the use of a microscope.

View Course

HIV Antibody Test Using A Rapid HIV Card

HIV antibody tests are specifically designed for routine diagnostic testing of adults; these tests are inespensive and extremely accurate. Most people develop detectable antibodies approximately 30 days after infection, although some seroconvert later.   The vast majority of people (99%) have detectable antibody by three months after HIV infection; a six month window is extremely rare with ...

View Course

Estimation of Haemoglobin Concentration

In this programme, the estimation of haemoglobin concentration is demonstrated in a video lesson. It describes the procedure in using the haemometer, a kit for determining haemoglobin and other laboratory tools in preparing a standard dilution.   Understanding the test procedure is critical to the interpretation of the test results.

View Course

Blood Glucose Monitoring

This pharmacological resource demonstrates the procedure in quatitative measuring of glucose level in the blood. The OneTouch Horizpon Test Strip with the OneTouch Horizon Blood Glusose meter will be used for this purpose.   These test strips are for in vtiro diagnostic use, that is, testing outside the body. The video demonstration covers the procedure form preparation to observing for res...

View Course

Malaria Antigen Rapid Test

Malaria is a disease caused by four identified spicies of Plasmodia, namely Plasmodium vivax, Plasmodium falciparum, Plasmodium ovale and Plasmodium malariae. It is transmitted by the female anopheles mosquito. It is a disease that can be treated in just 48hrs, yet it can cause fatal complications if the diagnosis and treatment are delayed. It is re-emerging as the the number one infectious kille...

View Course

One Step Pregnancy Dipstick Test

This pharmacological procedure demonstrates the procedure in a pregnancy test.  The One Step Pregnancy Test Strip would be used in the test, utilizing a combination of antibodies including a monoclonal hCG antibody to selectively detect elevated levels of hCG.

View Course

Barfoed's Test - Qualitative Test in Carbohydrates

Barfoed's test is a copper reduction test but unlike Benedict's test, it is carried out in an acid (pH 4.6) rather than alkaline solution. This video lesson demonstrates the procedure in the qualitative test for carbohydrates. Barfoed's test distinguishes monosaccharides from disaccharides. The procedure covers the description of the laboratory instruments and materials that are used in such test...

View Course

Seliwanoff's Test - Qualitative Test in Carbohydrates

This programme demonstrates in a video lesson the procedure in the qualitative test in carbohydrates using the Seliwanoff's Test. Seliwanoff's Test is a test to distinguish keto sugars form aldo sugars. Ketoses form furfurals more rapidly than aldoses. A compasison of the rate of colour formation is the bases of distinguishing between these two types of sugars.   At the end of the procedure...

View Course

Benedict's Test - Qualitative Test in Carbohydrates

This programme demonstrates in a video lesson, the procedure in the Qualitative test for Carbohydrates. In this Biochemistry Laboratory Test, the Benedict's test procedure is demonstrated. Benedict's test distinguishes reducing sugars from non reducing sugars. The procedure covers the description of the laboratory instruments and materials that are used in such tests.   At the end of the p...

View Course

Bial's Test - Qualitative Test in Carbohydrates

In this Biochemistry laboratory test, the Bial's Test procedure is demonstrated. The lesson covers the description of the laboratory instruments and materials that are used in such tests and the test procedure.   The objective of these experiments is to augment the understanding of basic principles of drug biological response. At the end of the lesson, students should be able to describe t...

View Course

Molisch's Test - Qualitative Test in Carbohydrates

Molisch's test is a test for carbohydrates in general. This test is useful for identifying any compound which can be dehydrated to furfural or hydroxymethyl furfural in the presence of H2SO4. The lesson will demonstrate the procedure in the Molisch's Test.   It also describes the laboratory instruments and materials that are used in such tests. The objective of the test is to describe the b...

View Course

Biuret's Test - Qualitative Test in Proteins

Some structural unit's characteristic of particular proteins enables these proteins to undergo various colour reactions with some reagents. We depend on these colour reactions to identify such proteins. The Biuret's test procedure is presented in this programme.   This video lesson demonstrates the laboratory procedure in Biuret's test. At the end of the lesson, students should be able to d...

View Course

Xanthoproteic's Test - Colour Reaction of Proteins

Xanthoprotein is a yellow acid substance formed by the action of hot nitric acid on albuminous or protein matter and is changed to a deep orange-yellow colour by the addition of ammonia. The colour reaction is used to identify such proteins in the Xanthoproteic Test.   This video lesson demonstrates the laboratory procedure in Xanthoproteic Test. At the end of the lesson, students should be...

View Course