Biuret's Test - Qualitative Test in Proteins

Overview

"Biuret_Test_2" courtesy of Ozone aurora. cc by

Some structural unit's characteristic of particular proteins enables these proteins to undergo various colour reactions with some reagents. We depend on these colour reactions to identify such proteins. The Biuret's test procedure is presented in this programme.

 

This video lesson demonstrates the laboratory procedure in Biuret's test. At the end of the lesson, students should be able to describe the test and its positive results, understand the basic principle and write a balanced equation for the test.

Author(s): George KoffuorView Details of Author
Year: 2012
Keywords: biuret's test, proteins, peptides, amino-acids, qualitative test
Licensing Condition:

cc-by-nc© 2012, KNUST

Unless otherwise noted, the content of this programme is licensed under the terms of a Creative Commons Attribution-NonCommercial 3.0 Unported License.

Disclaimer

 

Any information and/or video demonstration in this material is intended to inform and to educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. You should speak to your lecturer, healthcare professional or lab technician and make an appointment, if you have questions or concerns about this information.

 

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Introduction

 

Biuret's test for peptide bonds is a reaction characterised by a violet colour upon the addition of copper sulphate to all compounds with two amide or peptide bonds linked directly or through an intermediate carbon atom. It is used in the detection and estimation of proteins and peptides having more than two amino-acids.

A pink to purple/violet colour indicates the presence of proteins. A more intense coloration indicates the presence of more complex proteins.

 

Objectives

 

Write a balanced equation for Biuret's Test

Understand the principle behind Biuret's Test.

 

Lesson Content

 

Biuret's Test (video)
Biuret's Test

 

 

Credits

 

Author

gkoff

Dr. George Koffuor
Department of Pharmacology
College of Health Sciences - KNUST
Kumasi, Ghana

Student Volunteer


Stephen Fletchyann
Department of Communication Design
CASS - KNUST
Kumasi, Ghana

 

OER Technical Support

 

 

 

Benjamin Prempeh
OER Media Specialist
KNUST, Kumasi - Ghana
adobrog@yahoo.com

John Kofi Dogbey
Media Specialist
KNUST,Kumasi - Ghana
johndogbey@gmail.com


Voice Over

ella kasanga

Ella Kasanga
Kumasi, Ghana

 

Acknowledgment

 

This programme was produced with support from the following:

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