Seliwanoff's Test - Qualitative Test in Carbohydrates

Overview

This programme demonstrates in a video lesson the procedure in the qualitative test in carbohydrates using the Seliwanoff's Test. Seliwanoff's Test is a test to distinguish keto sugars form aldo sugars. Ketoses form furfurals more rapidly than aldoses. A compasison of the rate of colour formation is the bases of distinguishing between these two types of sugars.

 

At the end of the procedure, an observation of the test result is made and interpreted and a well balanced reaction equation is obtained.

Author(s): George KoffuorView Details of Author
Year: 2012
Keywords: Seliwanoff's test, aldo sugars, keto sugars,
Licensing Condition:

cc-by-nc© 2012, KNUST

Unless otherwise noted, the content of this programme is licensed under the terms of a Creative Commons Attribution-NonCommercial 3.0 Unported License.

Disclaimer

 

Any information and/or video demonstration in this material is intended to inform and to educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. You should speak to your lecturer, healthcare professional or lab technician and make an appointment, if you have questions or concerns about this information.

 

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Introduction

 

Seliwanoff's Test is a test to distinguish keto sugars form aldo sugars. Keto sugars dehydrate in the presence of concentrated acids to yield furfurals or their derivatives which reacts with resorcinol in Seliwanoff reagent to yield a cherry-red coloured complex seen as the positive result.

 

A cherry-red colour indicates the presence of ketohexoses. Ketopentoses yield blue-green solution whiles aldoses and disaccharides give no colour change.

 

Objectives

 

Distinguish between aldo sugars and keto sugars.

Outline the test process.

Write a well balanced reaction equation for the test.

 

Lesson Content

Seliwanoff's Test (video)
Seliwanoff's Test

Credits

 

Author

gkoff

Dr. George Koffuor
Department of Pharmacology
College of Health Sciences - KNUST
Kumasi, Ghana

Student Volunteer

christola

Christola Lord Acheampong
Department of Communication Design
CASS - KNUST
Kumasi, Ghana

 

OER Technical Support

 

 

 

Benjamin Prempeh
OER Media Specialist
KNUST, Kumasi - Ghana
adobrog@yahoo.com

John Kofi Dogbey
Media Specialist
KNUST,Kumasi - Ghana
johndogbey@gmail.com


Voice Over

ella kasanga

Ella Kasanga
Kumasi, Ghana

 

Acknowledgment

 

This programme was produced with support from the following:

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