Laboratory Methods for Clinical Microbiology

Overview

This interactive course deal with the principles of discriminative staining of microorganisms. it demostrates the procedure in performing a Gram stain, AFB stain, and Geimsa stain. The lessons explains how diagnostic tests are evaluated and understand the significance of the sensitivity, specificity, and predictive value of tests used in medical practice.

 

After studying this course, the studetn would be abke to know the various types of serological tests, the principles of the tests, and some practical applications of these assays

 

It also includes lessons on the principles of microbial antigen detection and the prinicple of polymerase chain reaction, including real-time PCR.

Author(s): Profs. Adu-Sarkodie, EnglebergView Details of Author
Year: 2009
Keywords: oer, laboratory methods, clinical microbiology
Licensing Condition:

cc-by-nc© 2009, KNUST

Unless otherwise noted, the content of this programme is licensed under the terms of a Creative Commons Attribution-NonCommercial 3.0 Unported License.

 

Disclaimer

 

Any medical information in this material is intended to inform and to educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. You should speak to your physician or make an appointment to be seen if you have questions or concerns about this information or your medical condition.

 

Note: Parts of this Programme are under construction

 

Introduction

 

Welcome to LABORATORY METHODS FOR CLINICAL MICROBIOLOGY!

This course contains lessons on methods for clinical microbiology with interactive videos and animations on microbiological stains and antibody-based identification of pathogens. In assessing the accuracy of diagnostic tests, there is a interactive tutorial available. You would also learn about measuring antibody response to infection, detection of microbial antigens and nucleic acid amplification (PCR).

 

Note: Parts of this Programme are under construction.

 

Objectives

 

Understand the principles of discriminative staining of microorganisms.

Be able to perform a Gram stain, AFB stain, and Geimsa stain.

Know how diagnostic tests are evaluated and understand the significance of the "sensitivity," "specificity," and predictive value" of tests used in medical practice.

Know the various types of serological tests, the principles of the tests, and some practical applications of these assays.

Understand the principles of microbial antigen detection.

Understand the prinicple of polymerase chain reaction, including real-time PCR.

 

Lesson Content

References

     

    There are no references for this course yet.

     

Credits

 

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Prof. Yaw Adu-Sarkodie, PhD,

Head, Department of Clinical Microbiology,

School of Medical Sciences,

KNUST, Kumasi, Ghana

 

 

 

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Prof. Cary Engleberg, M.D.,

Departments of Internal Medicine, 

and Microbiology & Immunology,

University of Michigan Medical School

 

 

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Mr. Charles Agyei Osei,

Technical Instructor,

Department of Clinical Microbiology

School of Medical Sciences,

KNUST, Kumasi, Ghana

 

Acknowledgment

 

This programme was produced with support from the following:

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